Composite

Part:BBa_K4147011:Design

Designed by: Ana Belem García González   Group: iGEM22_Tec-Chihuahua   (2022-10-08)


mCherry (RFP) construct with lacI regulated, lambda pL hybrid promoter for bacterial expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When synthetizing this part make sure you add some extra bp on both ends to facilitate the restriction process.


Source

The CDS for mCherry reporter was obtained from the registry under the accession code BBa_J06504.

References